Probiotic and Functional Properties of Strains

Analysis and optimization of functional properties of strain and strain collections:

Probiotic properties

With help of established assays we provide a time and cost-efficient support for the analysis and optimization of probiotic properties.

Established assays include:

Testing the resistance of strain(s) to simulated stomach and/or gut conditions

Testing the natural binding capacity of strain(s) to human intestinal cells such as Caco2, HT29, T84-cell models, etc.

Taxonomy analysis (Using MALDI-TOF, RAPD-Profiling, etc.)

Testing virulence factors (presence or absence of Plasmid, antibiotic resistance profile, factors observed in the genus or species etc.)

Testing in vivo properties (intrinsic immunogenicity etc.)

Analysis of functional properties

Beside basic probiotic properties, we provide customers with support for the analysis of specific functional properties.  Along previous projects we developed assay to:

Analyze anti-pathogenic properties e.g.

Expression of anti-pathogenic compounds (i.e. agar spot        assay, co-culturing assay and more)

Neutralization assay (co-aggregation assay, etc.)

In vivo infection and immunization challenge models

We have the ability to work with pathogens classified under risk class S2 and S3** according to German biological agents regulation

Analyze anti-cytotoxicity properties e.g.

Neutralization of cytotoxicity effect on human intestinal          cells such as Caco2 and HT29 cells.

Analyze immunomodulatory properties e.g.

Expression of antigen on the surface of bacteria

in vivo immunization

Bacteria antibody-specific quantification assay

Optimization of functional properties

Our experience has shown that specific selection process can help strongly improving the properties of any strain.

Using a combination of selection processes like High throughput specific sub-cloning, function specific isolation processes and targeted optimization of culture conditions; we help customers to improve the functional properties of their strains.

Please, discuss with us you requests and questions:  Contact Us